On November 23, 2012, the media revealed that it took only 45 days for a chicken raised in Shanxi Suhai Group to hatch and serve on the table. It was fed with feed and medicine. The Huangpu River dead pig incident: In March 2013, there were a large number of floating dead pigs in the waters of the Songjiang section of the Shanghai Huangpu River. The floating dead pigs appeared from the upper reaches of the Huangpu River. As a result, people are paying more attention to the detection of feed residues, veterinary drugs and other drug residues, preservatives, additives and microorganisms.
In the feed national standard catalog, the detection of feed microorganisms includes the total number of bacteria, Shigella, Salmonella, total mold, coliform, and Lactobacillus acidophilus. Among them, the detection of the total number of feed bacteria and molds requires counting the total number of colonies. Most companies now basically manually count with the naked eye, which is both time-consuming and labor-intensive. Xunshu automatic colony counter can help the experimenter to complete the counting of the total number of colonies, and improve the specifications of the enterprise microbiology laboratory.
1. Procedure for determination of total bacteria
2. Selection of the total number of plate colonies
Select the plate with the total number of bacteria between 3 and 300 as the standard for determining the total number of bacteria. The average number of colonies of two plates is used for each dilution. When one of the two plates has larger flaky colonies, it should not be used. The average number of plate colonies with flaky colonies should be taken as the dilution For the total number of bacteria, if the flaky colonies are less than half of the plate, and the other half of the colonies are distributed evenly, you can calculate half the plate and multiply it by 2 to represent the total number of bacteria in the whole plate.
3. Application of Xunshu automatic colony counter
Xunshu V series automatic colony counter is a series of cost-effective instruments specially designed for grassroots professional microbiology laboratory. The newly designed fully enclosed shooting, supplemented by full space, wide light band and suspended dark field device, completely solves the industry problem of affecting the statistical accuracy due to the interference of external light on the refracted light spot of the glass culture dish. Using ConlonfastTM colony intelligent identification technology, suitable for all kinds of complex petri dishes.
ConlonfastTM colony intelligent identification technology: to realize automatic colony counting, colony morphology analysis, adhesion colony segmentation, automatic color recognition, automatic impurity rejection, as well as a large number of advanced functions such as morphological analysis, automatic conversion and measurement
In the determination of the total number of feed bacteria, because the sample solution is made by grinding the feed, a certain amount of feed residue will remain in the culture medium. The culture medium residue is a problem in the colony statistics process. The instrument's ConlonfastTM colony intelligent identification technology, precise algorithm, impurity removal function, image processing and related parameter settings can well eliminate the influence of residues in the statistical process.
In the determination of the total number of feed bacteria, the national standard "Selection of the total number of plate colonies" mentioned "If the platelet colonies are less than half of the plate, and the other half of the colonies are distributed evenly, you can calculate half of the plate and multiply it by 2 to represent the bacteria The area selection statistics tool of "Total" software has: circle, rectangle, semicircle, fan shape and arbitrary shape, to realize the local statistics of colonies,
The instrument's database can automatically store plate pictures and colony statistics, which is convenient for follow-up and verification.
Hangzhou Xunshu Technology Co., Ltd. is a high-tech company specialized in microbial analysis and testing technology research and equipment production. At present, it mainly produces automatic colony counters, spiral inoculation microbial colony analyzers, bacteriostatic circle measuring instruments, β-lactam testers, antibiotic potency analyzers, algae counters, algae auxiliary identification systems, zooplankton counters, biological Microanalysis system and other products. Products are widely used in scientific research, inspection and quarantine, quality supervision, environmental monitoring, disease control center, drug and biological product verification, food and pharmaceutical daily chemical production, and iron and petrochemical chemical power.
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