Ancient Dot Bio Sharing: ELISA Test Method!
Our company is a high-tech biological company, which has long-term supply of various species of elisa kits. The product range is complete! The ELISA method has been widely used in scientific research. ELISA can be used to determine antigens and can also be used for antibody determination. There are three essential reagents in this method: (1) solid phase antigen or antibody, ie, "immunosorbent"; (2) enzyme-labeled antigen or antibody, called "enzyme conjugate", "conjugate" (conjugate); (3) a substrate for the enzymatic reaction. Depending on the source of the reagent and the condition of the specimen and the specific conditions of the assay, various types of assays can be devised. Ancient Duo Bio-sharing: What are the ELISA assays:
1. Indirect ELISA: For screening antibodies (specific antibodies against specific antigenic components). This method is the most common method for determining antibodies.
2. Sandwich ELISA: The amount used to detect the antigen of interest. This has the advantage of avoiding direct labeling of specific antibodies, but increasing the number of steps and assay times.
3. Competitive ELISA: A method used to determine the specificity of an antigen or the cross-reactive component in a sample to be tested in order to increase the specificity of the experiment. According to the principle of competitive binding between the labeled antigen and the same unlabeled antigen to be tested, it is mainly used for the determination of small molecule antigens.
4. ABS-ELISA method, ABS is an abbreviation of avidin biotin system. Avidin is a glycoprotein with a molecular weight of 60,000 and consists of four subunits that bind to biotin.
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