Other biochemical reagents (Human) Glycolic acid

Test principle of other biochemical reagents:

The CG kit is a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA). Standards with known CG concentrations and samples with unknown concentrations are added to microwell enzyme plates for detection. Incubate the CG and biotin-labeled antibodies first. After washing, HRP labeled with avidin was added. After incubation and washing, the unbound enzyme conjugate is removed, and then substrates A and B are added to act simultaneously with the enzyme conjugate. Produce colors. The color depth is proportional to the concentration of CG in the sample.

Content and preparation of other biochemical reagents

Kit components (stored at 2-8 ° C)

96-well configuration

48 hole configuration

Preparation

96/48 serving plate

1 board (96T)

Half plate (48T)

Ready-to-use

Plastic membrane cover

1 piece

Half block

Ready-to-use

Standard product: 8.0mg / L

1 bottle (0.6ml)

1 bottle (0.3ml)

Follow the instructions for sparse

Blank control

1 bottle (1.0ml)

1 bottle (0.5ml)

Ready-to-use

Standard dilution buffer

1 bottle (5ml)

1 bottle (2.5ml)

Ready-to-use

Biotin-labeled anti-CG antibody

1 bottle (6ml)

1 bottle (3.0ml)

Ready-to-use

Streptavidin-HRP

1 bottle (10ml)

1 bottle (5.0ml)

Ready-to-use

Wash buffer

1 bottle (20ml)

1 bottle (10ml)

Dilute according to the instructions

Substrate A

1 bottle (6.0ml)

1 bottle (3.0ml)

Ready-to-use

Substrate B

1 bottle (6.0ml)

1 bottle (3.0ml)

Ready-to-use

Stop solution

1 bottle (6.0ml)

1 bottle (3.0ml)

Ready-to-use

Specimen diluent

1 bottle (12ml)

1 bottle (6.0ml)

Ready-to-use

Other biochemical reagent materials

1. ? Distilled water.

2. ? Sampler: 5ul, 10ul, 50ul, 100ul, 200ul, 500ul, 1000ul.

3. ?

4. ? Oscillator and magnetic stirrer.

safety

1. • Avoid direct contact with the stop solution and substrates A and B. Once exposed to these liquids, rinse with water as soon as possible.

2. • Do not eat, drink, smoke or use cosmetics during the experiment.

3. ? Do not use your mouth to absorb any components in the kit.

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