New application fields of genome engineering

Through ingenious gene manipulation behavior, researchers at the American Craig Venter Institute transplanted a bacterial genome into yeast, and then transformed it into a hollow bacterial shell, resulting in a new microorganism. This technology provides an easier way to genetically modify organisms that are rarely studied in the laboratory, and is also of great value for the cultivation of microorganisms that produce fuel or remove toxic chemicals.

Thanks to years of research on microorganisms such as yeast and E. coli, scientists have been able to use these genetic tools to perform more complex genetic modification, such as replacing the entire chemical path to create microorganisms that can perform more complex tasks or produce substances more efficiently. . However, many microorganisms in the industry, such as those with unique ability to produce chemicals, are not easy to make. Scientists hope to be able to design targeted organic matter like photosynthetic microorganisms to more efficiently convert light into fuel.

By inserting the genomes of these bacteria into yeast, researchers at the Venter Institute found that they could manipulate them more easily. Researchers say that what people want is the ability of yeast or E. coli, not the organs that have their photosynthesis. It will be extremely interesting to combine these two genomes in the biofuel world.

The Venter Institute seeks to create life from scratch, and various technologies are constantly emerging from it. They want to create a synthetic gene and then use it to control or restart a receptor cell. In 2007, the institute published a paper describing its research results in genome transplantation. Genome transplantation is the transfer of a type of bacterial genome to a closely related bacterium, thus giving the host the characteristics of a donor. Last year, researchers created a synthetic genome by stitching together pieces of synthetic DNA.

In order to create a synthetic organism, the researchers had to transplant the synthetic genome into a cell to successfully restart the cell. The synthetic genome is assembled into yeast, which means it lacks some bacterial biomarker features. The researchers found that without these markers, the host bacteria would treat the transplanted genome as a foreign invader and destroy it.

A new technology published online in the "Science" magazine solved this problem. For the first time, the researchers transplanted the mycoplasma filamentous genome into yeast. Although scientists have grown bacterial DNA fragments in yeast, this is the first time that a complete bacterial genome has been grown in this way. Using existing yeast genetic engineering tools, the researchers chemically changed the genetic material of the bacteria so that it carried the molecular marker characteristics of the bacteria. The researchers transplanted this modified genome into mycoplasma goat (with a close relationship with M. filamentous) to produce a M. filamentous cell.

Jim Collins, a biological engineer at Boston University in the United States, said that this research has enhanced the ability of genome engineering and opened up new fields of application. For the bioenergy and biomaterials industry, this is a major step forward.

Researchers are currently working on testing other bacteria to transfer production technology to organic matter more relevant to the biofuel industry. Researchers say that the genetic pathways of organic matter can decompose environmental pollutants. After being genetically engineered into bacteria, they can survive in harsh and contaminated environments such as acid ponds, which can then be used to clean up these areas

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