What should I pay attention to during the preparation of the medium?
To configure the medium you need:
1. Prepare different media according to the nutritional needs of different microorganisms.
2, pay attention to the concentration of various nutrients and the appropriate ratio, to maintain the appropriate osmotic pressure.
3. Control the pH of the medium within a suitable range to facilitate the growth and reproduction of different types of microorganisms or the accumulation of metabolites.
4, should pay attention to the ratio of various raw materials, the appropriate ratio of each medium, can be done according to the teacher's requirements.
5. Pay attention to the time when you need to heat up.
step:
(1) Accurate weighing reagents: According to different fungi and uses, select the appropriate medium, and the reagents required for the medium must be pure.
(2) Correcting the pH value: Put the various components of the weighed medium into the container, mark the scribe line, heat and dissolve, add water, measure the pH, and measure with a precision test paper or a pH meter with a pH of 6.8 to 8.0. The pH was adjusted to the appropriate range with 1 N NaOH and 1 N HCl.
(3) Filtration: The glass funnel is placed on an iron frame, and the cotton is placed in a gauze or placed in a funnel with a filter paper, and the above medium is poured therein to be filtered to be transparent.
(4) Packing: Dispense the filtered medium into a medium test tube or a triangular flask (5 mL per tube in the test tube; 100-150 ml in a triangular flask), stuff the cotton plug with kraft paper, and prepare for sterilization. .
(5) Sterilization: Sterilization of the medium, usually by high pressure steam sterilization. Generally, the vegetative cells of microorganisms are killed after being boiled in water, but the germ cells of the bacteria have strong heat resistance and must be autoclaved to achieve the purpose of thorough sterilization. The principle that the steam temperature rises as the pressure rises, that is, the higher the pressure, the higher the steam temperature. Therefore, under the same temperature conditions, the use of high pressure steam sterilization is better than dry heat sterilization. Moreover, in the case of damp heat, after the cells absorb water, the protein is easily coagulated and denatured, because the penetration of steam is strong and the sterilization effect is good.
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