The chromatographic fingerprint of Radix Isatidis was established by high performance liquid chromatography. A reversed-phase C18 column was used, and the mobile phase was water: methanol (VPV = 96: 4); the detection wavelength was 260 nm; and the flow rate was 110 mLPmin. Based on the relative retention value α and the relative area Sr, the chromatographic fingerprints were analyzed and compared, and a fast and simple chromatographic fingerprint analysis method for identifying Radix isatidis samples was established. This method provides more comprehensive information for the identification of traditional Chinese medicine samples and opens up new applications for HPLC in the field of sample analysis and identification of complex components.
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